A novel human cytochrome P4S0 gene (P450IIB): chromosomal localization and evidence for alternative splicing
Open Access
- 11 July 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 16 (13), 5783-5795
- https://doi.org/10.1093/nar/16.13.5783
Abstract
We have isolated from a single human liver cDNA library two clones which are highly homologous (78% over the coding region) to the major phenobarbital-inducible P450 from rat (P450IIB1). This is the first direct demonstration of the presence of the P450IIB gene subfamily in humans. This subfamily is much less extensive than the rodent homologues, but does appear to contain at least two genes. Of the cDNA clones isolated one is apparently normally spliced, whereas the other lacks exon 8 and retains all or part of intron 5. Both clones contain transcribed Alu sequences. The human P450IIB gene has been located to chromosome 19q12 19ql3.2 using a probe derived from intron 5, and is close to the CYP 2A locus encoding cytochrome P450IIA2. Restriction fragment length polymorphisms have been found with the enzymes BamHI and MSpI which will enable linkage to be determined between these two loci.This publication has 40 references indexed in Scilit:
- Chromosomal assignment of the gene encoding the human tissue inhibitor of metalloproteinases to Xp11.1–p11.4Annals of Human Genetics, 1987
- Linkage between the loci for peptidase D and cytochrome P-450 (CYP1) on chromosome 19Annals of Human Genetics, 1987
- Chromosomal assignment of c-MEL, a human transforming oncogene, to chromosome 19 (p13.2-q13.2)Somatic Cell and Molecular Genetics, 1986
- Regional localization of a human cytochrome P-450 (CYP1) to chromosome 19q 13.1?13.3Annals of Human Genetics, 1986
- Isolation and characterization of a novel cytochrome P-450-like pseudogeneBiochemical and Biophysical Research Communications, 1986
- The molecular mechanisms of two common polymorphisms of drug oxidation-evidence for functional changes in cytochrome P-450 isozymes catalysing bufuralol and mephenytoin oxidationXenobiotica, 1986
- A cytochrome P‐450 gene family mapped to human chromosome 19Annals of Human Genetics, 1985
- Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencingJournal of Molecular Biology, 1980
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- Screening λgt Recombinant Clones by Hybridization to Single Plaques in SituScience, 1977