Copper(II)-Quenched Oligonucleotide Probes for Fluorescent DNA Sensing
- 1 October 2004
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 126 (42), 13626-13627
- https://doi.org/10.1021/ja047252a
Abstract
A copper(II)-quenched molecular beacon was prepared by attaching fluorescein to the 3‘-end and a copper(II) complex to the 5‘-end of DNA. In the presence of complementary DNA, copper(II) and dye are spatially separated in the duplex and fluorescence increases up to 15-fold, with excellent discrimination of single base mismatches.Keywords
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