Cytotoxins against a Granulocyte Antigen System: Detection by a New Method Employing Cytochalasin‐B‐Treated Cells1
- 1 October 1977
- journal article
- research article
- Published by Wiley in Vox Sanguinis
- Vol. 33 (4), 206-211
- https://doi.org/10.1111/j.1423-0410.1977.tb04465.x
Abstract
A method for demonstrating granulocyte cytotoxins using a modified microdroplet dye exclusion technique and cytochalasin-B-treated cells is described. Cytochalasin-B-treated granulocytes were reacted against 223 sera from multitransfused pregnant and renal transplant patients. Incidence of granulocyte cytotoxins was 11.2%. Analysis (2times2 contingency tables) of the reactivity of GCT + sera showed highly significant (p<0.001) positive associations among 5 sera and a significant (p<0.001) negative association with another serum. The data are consistent with the hypothesis that a granulocyte antigen system is detected by cytotoxins and that granulocyte antigens ‘Gr1’ and ‘Gr2’ may be products of allelic genes.This publication has 8 references indexed in Scilit:
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