Inhibition of δ-Protein Kinase C Protects Against Reperfusion Injury of the Ischemic Heart In Vivo

Abstract

Background— Current treatment for acute myocardial infarction (AMI) focuses on reestablishing blood flow (reperfusion). Paradoxically, reperfusion itself may cause additional injury to the heart. We previously found that δ-protein kinase C (δPKC) inhibition during simulated ischemia/reperfusion in isolated rat hearts is cardioprotective. We focus here on the role for δPKC during reperfusion only, using an in vivo porcine model of AMI. Methods and Results— An intracoronary application of a selective δPKC inhibitor to the heart at the time of reperfusion reduced infarct size, improved cardiac function, inhibited troponin T release, and reduced apoptosis. Using ³¹P NMR in isolated perfused mouse hearts, we found a faster recovery of ATP levels in hearts treated with the δPKC inhibitor during reperfusion only. Conclusions— Reperfusion injury after cardiac ischemia is mediated, at least in part, by δPKC activation. This study suggests that including a δPKC inhibitor at reperfusion may improve the outcome for patients with AMI.