MORPHOLOGICAL REACTION OF SPERMATOZOA IN THE FEMALE REPRODUCTIVE TRACT OF THE RABBIT
- 1 October 1963
- journal article
- research article
- Published by Bioscientifica in Reproduction
- Vol. 6 (2), 245-255
- https://doi.org/10.1530/jrf.0.0060245
Abstract
A study of rabbit ejaculate and epididymal spermatozoa, and of spermatozoa recovered from various regions of the female tract, has revealed some consistent morphological differences in the state of the acrosome between these groups. Few acrosomes were missing or changed in epididymal or ejaculate spermatozoa. On the other hand, the acrosome was absent in spermatozoa released from the perivitelline space of fertilized ova. Uterine and tubal spermatozoa recovered 9 to 11 hr after insemination of ejaculates into oestrous or pseudopregnant females showed change or loss of the acrosome in a variable percentage (8 to 58%). The percentage acrosome loss from uterine samples taken after insemination of aliquots from the same ejaculate into pairs of females was closely similar within each pair, though variation occurred between pairs. Consistently higher acrosome losses (mean, 33%) were found following insemination of ejaculates collected after 1 month''s sexual rest than were found after insemination of the third ejaculate from each of the same males (mean. 17.2%). Considerably fewer acrosomes were missing from uterine spermatozoa recovered after insemination of epididymal spermatozoa (mean, 5%) compared to the loss after insemination of ejaculate spermatozoa (mean, 27.4%) taken previously from the same males. Fertility trials with ejaculate and epididymal spermatozoa showed no difference in fertilization rate, or in numbers of supplementary spermatozoa, in favour of the ejaculate samples, i.e. those which showed the greater percentage acrosome changes. In conclusion it is suggested that the quantitative variation in acrosome reaction between samples of uterine spermatozoa reflects differences in the samples, rather than differences in the female environment. The acrosome changes in the uterine and tubal spermatozoa of the domestic rabbit are probably not a morphological concomitant of capacitation, but more probably reflect impending sperm senility.Keywords
This publication has 6 references indexed in Scilit:
- Role of the rodent acrosome and perforatorium in fertilizationProceedings of the Royal Society of London. B. Biological Sciences, 1958
- CAPACITATION OF RABBIT SPERMATOZOA IN THE UTERUS WITH SPECIAL REFERENCE TO THE REPRODUCTIVE PHASES OF THE FEMALE1Endocrinology, 1958
- STUDIES ON THE ACROSOME. II. ACROSOME REACTION IN STARFISH SPERMATOZOAThe Biological Bulletin, 1954
- Fertilizability of rabbit ova and the effects of temperature in vitro on their subsequent fertilization and activation in vivoJournal of Experimental Zoology, 1952
- STUDIES ON THE ACROSOME. I. REACTION TO EGG-WATER AND OTHER STIMULIThe Biological Bulletin, 1952
- Observations on the Penetration of the Sperm into the Mammalian EggAustralian Journal of Biological Sciences, 1951