Chromosomal and phenotypic stability during regeneration of whole plants from tissue cultures of Brachycome dichromosomatica (2n=4)

Abstract

A system has been established for the regeneration of flowering plants from callus cultures of B. dichromosomatica, an Australian composite characterized by its diploid complement of only four chromosomes. After a year of subculturing, the callus will still give rise to plants which are perfect diploids and which exhibit distinctive chromosomal and phenotypic markers originally observed in the plants from which callus lines had been derived.