Isolement d'un nouveau ceto‐acide, produit a partir de ϵ‐N‐trimethyllysine par Neurospora crassa

Abstract

A new ϵ-N-trimethyllysine metabolite has been isolated from the mycelium of Neurospora crassa. The labelled compound produced from incubations in vivo and in vitro from ϵ-N-trimethyl ([¹⁴C]H₃)L-lysine has been identified as 2-keto-ϵ-N-trimethyl-hexanoic acid by reducing its 2,4-dinitrophenyl hydrazone back to ϵ-N-trimethyllysine by hydrogenolysis in a Parr bomb. Analyses on TLC and in four different ion exchange chromatographic systems show the appearance of a ninhydrin positive product having the same R _f and the same retention time as ϵ-N-trimethyllysine; it contains more than 85% of the radioactivity of the 2,4-dinitrophenylhydrazone of the keto acid.