A Rapid Vapor-Phase Acid (Hydrochloric Acid and Trifluoroacetic Acid) Hydrolysis of Peptide and Protein
- 1 December 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 102 (6), 1593-1597
- https://doi.org/10.1093/oxfordjournals.jbchem.a122209
Abstract
A new method for the acid hydrolysis of protein is presented. Peptide bonds are cleaved by the action of an HGl/trifluoroacetic acid (TFA) vapor mixture. Contamination for the hydrolysis mixture is reduced to low levels (1–3 pmol). Recovery of hydrophobic amino acid is improved. Short reaction times are achieved and rapid removal of acids is facilitated. The reaction temperature is 158°C for reaction times of 22.5 and 45 min with 7 M HC1 and 10% TFA containing 0.1 % phenol.This publication has 5 references indexed in Scilit:
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