Method for Isolating Single Cells and Preparation of Clones from Human Bone Marrow Cultures.

Abstract

A simple method for isolating single tissue culture cells had been developed. This technique involves placing small glass squares in a Petri dish, adding a suspension of cells to the dish, and allowing the cells to settle and adhere. The individual glass squares are then removed and observed microscopically, and those squares containing a single cell are placed in culture vessels. By this procedure, single, healthy-appearing cells may be selected and placed in a variety of cultural conditions in order to determine those which are optimum or necessary for multiplication.