Phosphatidylserine Synthesis in Castor Bean Endosperm

Abstract

Phosphatidylserine synthesis by the endoplasmic reticulum fraction isolated from castor bean (Ricinus communis var. Hale) endosperm was assayed by measuring the incorporation of 14C-l-serine into chloroform-soluble material. Both phosphatidylserine and phosphatidylethanolamine were identified as products. The incorporation required calcium ions and showed an optimum pH of 7.8 in 2 mm CaCl2. Phosphatidylethanolamine and CDP-diglyceride stimulated the reaction only about 40 to 50% and primary alcohols had relatively little effect on the incorporation. These and other results suggest the synthesis of phosphatidylserine in this tissue occurs by an exchange reaction but the relative roles of phospholipase D and phosphatidylethanolamine: l-serine phosphatidyltransferase remain to be elucidated.