STRUCTURAL STUDIES ON INDUCED ANTIBODIES WITH DEFINED IDIOTYPIC SPECIFICITIES .3. N-TERMINAL AMINO-ACID SEQUENCE OF HEAVY AND LIGHT-CHAINS OF MOUSE ANTI-STREPTOCOCCAL ANTIBODIES - A5A, S8, AND S117

Abstract

The murine VH [H chain variable region] genetic map consists of 2 different subloci of VH genes. Seven idiotypes were allocated to these 2 different subloci, 1 of which contains coding for the A5A idiotype; the 2nd contains the genes encoding for 6 other idiotypic markers. Previous studies showed that anti-Ars antibodies are best classified in the murine VHII subgroup. Other studies indicate that the J558 idiotype could be classified in a similar fashion. The sequence of the T15 myeloma protein VH region is most characteristic of the murine VHII subgroup. These studies were undertaken to study the VH subgroup of 2 additional murine VH markers. Since 1 of these markers was allocated to a separate sublocus, comparisons can be made between the framework residues (VH subgroup) and the murine genetic map. Within a single presently defined sublocus, proteins exist which contain remarkably different framework residues.