A New Elution Procedure Using Chloroform, a Nonflammable Organic Solvent

Abstract

A new method for eluting [human] red cell antibodies using chloroform to be effective. The method is similar to ether and xylene techniques but can be completed within 10 min after adequate cell washing. Comparison studies using ether, xylene and chloroform showed that antibodies eluted by chloroform yielded equivalent titration scores. Antibodies within the Ss blood group system were easily eluted using chloroform but not using ether. The chloroform method yielded informative eluates when prepared from red cells of patients with warm antibody autoimmune hemolytic anemia, drug-induced immune hemolytic anemia, hemolytic disease of the newborn caused by ABO or Rh fetal-maternal incompatibility, or from patients having a positive direct antiglobulin test as a result of alloantibodies stimulated by recent transfusion (delayed transfusion reaction). The advantages of chloroform elution are: chloroform is nonflammable; the eluate is readily obtained from the top layer after centrifugation; no residual solvent remains in the eluate; and the method is rapid.