Pyrolysis Mass Spectrometry as a Method for Inter-strain Discrimination of Candida albicans

Abstract

A claim that Candida albicans strains NCPF 3153 and B311 were identical was investigated. Authentic strains were shown to be distinct (P < 0.1%) by pyrolysis mass spectrometry (Py-MS). Of twelve strains, provided as clones of NCPF 3153, seven were authenticated, one yielded an equivocal result and four were distinct from both NCPF 3153 and B311. Of eight B311 clones, six were authenticated and two yielded equivocal results. Although five non-C. albicans yeast strains were identified as distinct from B311 and NCPF 3153, Torulopsis glabrata NCPF 3240 was identified as B311, and one clinical isolate of C. albicans as NCPF 3153. This could be explained by the specificity of the mathematical analysis for discrimination between the authentic strains.