Transients in the Photosynthetic Carbon Reduction Cycle Produced by Iodoacetic Acid and Ammonium Chloride

Abstract

Changes in ¹⁴C content of metabolites in photosynthesising Chlorella have been investigated following the addition of iodoacetic acid or ammonium chloride. The ¹⁴C content of phosphoglyeric acid increases in the former and decreases in the latter case. Both reagents divert ¹⁴C from sucrose and polysaccharide into compounds of and associated with the tricarboxylic acid cycle. The principal site of iodoacetic acid inhibition is triose phosphate dehydrogenase whereas ammonium chloride stimulates the conversion of phosphoglyceric acid to phosphoenolpyruvate and pyruciv acid. The latter suggests a direct effect of the ammonium ion on phosphoglyceromutase, enolase, or pyruvic kinase in vivo. Changes in the concentration of alanine, aspartic acid, glutamic acid, serine, and glutamine upon addition of the ammonium ion are not well correlated with their ¹⁴C labelling patterns.