ISOLATION AND SEQUENCE ANALYSIS OF A PIGMENT-DISPERSING HORMONE FROM EYESTALKS OF THE CRAB,CANCER MAGISTER
Open Access
- 1 February 1986
- journal article
- research article
- Published by University of Chicago Press in The Biological Bulletin
- Vol. 170 (1), 135-143
- https://doi.org/10.2307/1541386
Abstract
The application of separation procedures, including liquid partition, gel filtration, ion-exchange chromatography, partition chromatography, and reversed-phase high-performance liquid chromatography, enabled the purification of an octadecapeptide pigment-dispersing hormone (PDH) from powdered lyophilized eyestalks ofthe crab, Cancer magister. Automated Edman degradation, followed by the identification of carboxyl-terminal amide, established the sequence of the peptide as: Asn-Ser-Glu-Leu-Ile-Asn-Ser-Ile-Leu-Gly-Leu-Pro-Lys-Val-Met-Asn-Asp-Ala-NH2. The characterized Cancer PDH is structurally identical to an octadecapeptide PDH of Uca pugilator, but differs from the PDH of Pandalus borealis at positions 3, 4, 11, 13, 16, and 17.This publication has 13 references indexed in Scilit:
- Peptides in the sinus gland ofCardisoma carnifex: isolation and amino acid analysisJournal of Comparative Physiology B, 1983
- Structure-activity relationships of a pigment-dispersing crustacean neurohormonePeptides, 1982
- The purification and partial characterization of the melanophore-dispersing hormone of Uca pugilatorGeneral and Comparative Endocrinology, 1980
- Comparative electrophoretic studies of crustacean neurosecretory hyperglycemic and melanophore-stimulating hormones from isolated sinus glandsJournal of Comparative Physiology B, 1977
- Structure of locust adipokinetic hormone, a neurohormone that regulates lipid utilisation during flightNature, 1976
- Structure of a light-adapting hormone from the shrimp, Pandalus borealisBiochimica et Biophysica Acta (BBA) - Protein Structure, 1976
- A proposed uniform method of reporting response values for crustacean chromatophorotropins: the standard integrated responseCellular and Molecular Life Sciences, 1967
- Neurosecretory Control of Pigmentary Effectors in CrustaceansAmerican Zoologist, 1966
- Separation of neurosecretory pigmentary-effector hormones of the crustacean eyestalkGeneral and Comparative Endocrinology, 1965
- The pigmentary effector system. VI. The dual character of endocrine co-ordination in amphibian colour changeProceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character, 1931