Ribonuclease of Bacillus Subtilis

Abstract

The occurrence of microbial ribonuclease (RNase) has been reported by several workers (1–4), but most of the preparations reported so far except the RNase T₁ from Takadiastase by Sato and Egami (5) were not so much purified. RNase of Bacillus subtilis was accumulated in a medium as well as amylase and protease. A 400 fold purification was achieved by acid treatment, ammonium sulfate fractionation and column chromatography. The results indicated that there were two types of RNases in a culture medium of B. subtilis. The present communication deals with the separation, characterization and partial purification of RNase of B. subtilis.