The flaFIX gene product of Salmonella typhimurium is a flagellar basal body component with a signal peptide for export
Open Access
- 1 April 1987
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 169 (4), 1493-1498
- https://doi.org/10.1128/jb.169.4.1493-1498.1987
Abstract
FlaFIX, the structural gene for the periplasmic P ring of the flagellar basal body of Salmonella typhimurium, was cloned. Two gene products with apparent molecular weights of 38,000 and 40,000 were identified by minicell analysis. Data from pulse-chase and membrane fractionation experiments and data on the inhibitory effect of the proton ionophore carbonyl cyanide m-chlorophenylhydrazone all indicated that the 40-kilodalton protein was a precursor form which, after export across the cytoplasmic membrane accompanied by cleavage of a signal peptide, gave rise to the mature protein in the periplasm. The N-terminal amino acid sequence of the FlaFIX protein, predicted from the DNA sequence, conformed well to known signal peptide sequences. The results indicate that the P-ring protein of the basal body (unlike flagellin and possible some other external flagellar components) crosses the cytoplasmic membrane in a conventional signal peptide-dependent manner.This publication has 38 references indexed in Scilit:
- Covalent structure of three phase-1 flagellar filament proteins of SalmonellaJournal of Molecular Biology, 1985
- PROTEIN SECRETION IN ESCHERICHIA COLIAnnual Review of Microbiology, 1985
- GENETIC ANALYSIS OF PROTEIN EXPORT IN ESCHERICHIA COLI K12Annual Review of Biochemistry, 1985
- Export and secretion of proteins by bacteriaFEMS Microbiology Letters, 1985
- Genetic Analysis of Three Additional fla Genes in Salmonella typhimuviumMicrobiology, 1984
- Bacterial Motility and the Bacterial Flagellar MotorAnnual Review of Biophysics and Bioengineering, 1984
- A system for shotgun DNA sequencingNucleic Acids Research, 1981
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Bacterial Flagella: Polarity of ElongationScience, 1970
- Polarity of Flagellar Growth in SalmonellaJournal of General Microbiology, 1969