Mediation of Macrophage Cytolytic and Phagocytic Activities by Antibodies of Different Classes and Class-Specific Fc-Receptors
- 31 July 1977
- journal article
- research article
- Published by The American Association of Immunologists in The Journal of Immunology
- Vol. 119 (2), 367-373
- https://doi.org/10.4049/jimmunol.119.2.367
Abstract
The classes of antibodies that mediate the phagocytosis and cytolysis of 51Cr-labeled chicken erythrocytes by IC-21 macrophages, an established line of mouse peritoneal macrophages, were identified. The phagocytic activity of IC-21 macrophages, as determined by a functional inhibition assay with mouse myeloma proteins, depended mainly on IgM and IgG2a antibodies and to a lesser extent on IgG2b antibodies. Extracellular cytolysis of target cells was mediated solely by IgG2b antibodies. These results correlate with the previously documented specificities of discrete Fc-receptors for IgG2a and IgG2b immunoglobulins on IC-21 cells. Thus, phagocytosis and cytolysis appear to be mediated by antibodies of different classes operating through separate and distinct sites on the surface of IC-21 macrophages.This publication has 3 references indexed in Scilit:
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- Red Cells Coated with Immunoglobulin G: Binding and Sphering by Mononuclear Cells in ManScience, 1967
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951