Evidence of Gene Diminution During the Formation of the Macronucleus in the Protozoan, Stylonychia

Abstract
The course of events by which a macronucleus is formed from a micronucleus after conjugation in the ciliated protozoan, Stylonychia, suggests that genetic diminution may occur. This idea is supported by determinations of the density profiles and melting curves for micro- and macronuclear DNAs. Macronuclear DNA consists of a single density component and melts as if it were a single component. Micronuclear DNA consists of four or more density components and melts as if it were a mixture of several DNAs of different base compositions. These data indicate that at least 60% of the micronuclear DNA components are absent from the macronuclear DNA.