The Effect of Tissue Ageing on Intestinal Uptake of Amino-Acids in Vitro
- 1 January 1966
- journal article
- research article
- Published by S. Karger AG in Digestion
- Vol. 105 (1), 17-26
- https://doi.org/10.1159/000202016
Abstract
The gradual loss of viability of intestinal tissue was demonstrated in the course of various ageing procedures. It was found to be inadvisable to incubate a fragment of tissue for more than 3 hrs, or to keep it in vitro for more than 3 hrs before use. By measuring the changes in the various components of entry into intestinal tissue during the course of ageing, a test for the functional viability of intestinal tissue based on the uptake of amino-acid in the absence and presence of 2:4-dinitrophenol was validated.Keywords
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