5' splice site selection in yeast: genetic alterations in base-pairing with U1 reveal additional requirements.
Open Access
- 1 October 1988
- journal article
- research article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 2 (10), 1258-1267
- https://doi.org/10.1101/gad.2.10.1258
Abstract
Using a strategy of compensatory nucleotide changes between yeast U1 and a 5' splice site, we have analyzed the contribution of base-pairing to the efficiency and fidelity of pre-mRNA splicing in vivo. Watson-Crick base-pairing interactions with U1 can be demonstrated at intron positions 1 and 5 but not at position 4. Moreover, restoration of the ability to pair with U1 is not sufficient to restore activity in the second step of splicing to intron position 1 mutants. Finally, in contrast to recent observations in mammalian systems, we find that the precise position of 5' splice site cleavage is not determined solely by the base-pairing interaction with U1. Rather, the presence of a G residue at position 5 is required for the correct localization of the nucleolytic event. Taken together, these results indicate that the demands for 5' splice site selection and utilization are more complex than a simple maximization of Watson-Crick interactions with U1.This publication has 39 references indexed in Scilit:
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