Factors Affecting thein vitroMetabolism of R-(+)-and S-(-)-Nicotine by Guinea-pig Liver Preparations
- 1 January 1973
- journal article
- research article
- Published by Taylor & Francis in Xenobiotica
- Vol. 3 (9), 563-572
- https://doi.org/10.3109/00498257309151544
Abstract
1. Cotinine and the diastereoisomeric nicotine-1-N-oxides were the major in vitro metabolites of R-(+)- and S-(-)-nicotine produced by various guineapig hepatic preparations; metabolism was greatest in the 10 000 × g supernatant fraction, being enzymic and NADP(H) dependent. The effect of various substrate concentrations and incubation times on metabolism in the 10 000 × g supernatant fraction was investigated. 2. The ratio of the diastereoisomeric N-oxides formed was markedly stereoselective but the formation of cotinine and disappearance of nicotine were not. 3. Cotinine formation, but not the disappearance of nicotine or formation of nicotine-1′-N-oxide, was inhibited by SKF 525A and KCN.Keywords
This publication has 14 references indexed in Scilit:
- Species Variation in the Metabolism of R-(+)- and S-(-)-Nicotine by α-C- andN-Oxidationin vitroXenobiotica, 1973
- Characterization of the Diastereoisomers of Nicotine-1′-N-Oxide, a Metabolite of Nicotine, and Other Possible Oxidation Products by Nuclear Magnetic Resonance SpectroscopyXenobiotica, 1973
- A possible relation between pKa1 and lipid solubility and the amounts excreted in urine of some tobacco alkaloids given to manJournal of Pharmacy and Pharmacology, 1972
- The analysis of nicotine-1‘-N-oxide in urine, in the presence of nicotine and cotinine, and its application to the study of in vivo nicotine metabolism in manJournal of Pharmacy and Pharmacology, 1971
- Preparation of active, drug-metabolizing, microsomal enzymes under optimal conditions and by iso-electric precipitationJournal of Pharmacy and Pharmacology, 1970
- The metabolism of nicotine into two optically-active stereoisomers of nicotine-1′-oxide by animal tissues in vitro and by cigarette smokersBiochemical Pharmacology, 1970
- The Metabolism of Nicotine and Cotinine by a Mouse Liver PreparationActa Physiologica Scandinavica, 1970
- Effect of SKF 525-A on the excretion of amine oxides by the ratLife Sciences, 1968
- Some Characteristics of an Amide‐Hydrolysing Microsomal Enzyme before and after its Solubilization.Acta Pharmacologica et Toxicologica, 1961
- γ-(3-PYRIDYL)-γ-METHYLAMINOBUTYRIC ACID AS A URINARY METABOLITE OF NICOTINE1Journal of the American Chemical Society, 1957