Incorporation and metabolism of exogenous GM1 ganglioside in rat liver
- 1 July 1986
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 237 (1), 147-155
- https://doi.org/10.1042/bj2370147
Abstract
The pathways of metabolic processing of exogenously administered GM1 ganglioside in rat liver was investigated at the subcellular level. The GM1 used was 3H-labelled at the level of long-chain base ([Sph(sphingosine)-3H]GM1) or of terminal galactose ([Gal3H]GM1). The following radioactive compounds, derived from exogenous GM1, were isolated and chemically characterized: gangliosides GM2, GM3, GD1a and GD1b {nomenclature of Svennerholm [(1964) J. Lipid Res. 5, 145-155] and IUPAC-IUB Recommendations [(1977) Lipids 12, 455-468]}; lactosylceramide, glucosylceramide and ceramide; sphingomyelin, GM2, GM3, lactosylceramide, glucosylceramide and ceramide, relatively more abundant shortly after GM1 administration, were mainly present in the lysosomal fraction and reflected the occurrence of a degradation process. 3H2O was also produced in relevant amounts, indicating complete degradation of GM1, although no free long-chain bases could be detected. GD1a and GD1b, relatively more abundant later on after administration, were preponderant in the Golgi-apparatus fraction and originated from a biosynthetic process. More GD1a was produced starting from [Sph-3H]GM1 than from [Gal-3H]GM1, and radioactive GD1b was present only after [Sph-3H]Gm1 injection. This indicates the use of two biosynthetic routes, one starting from a by-product of GM1 degradation, the other implicating direct sialylation of GM1. Both routes were used to produce GD1a, but only the first one for producing GD1b. Sphingomyelin was the major product of GM1 processing, especially at the longer times after injection, and arose from a by-product of GM1 degradation, most likely ceramide.This publication has 38 references indexed in Scilit:
- GM2-ganglioside metabolism in cultured human skin fibroblasts: unambiguous diagnosis of GM2-gangliosidosisBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1985
- Effect of drugs and temperature on biosynthesis and transport of glycosphingolipids in cultured neurotumor cellsBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1984
- A method for rapid isolation of rough and smooth microsomes and golgi apparatus from rat liver in the same sucrose gradientExperimental Cell Research, 1980
- THE SUBCELLULAR LOCALIZATION OF ACETYLCHOLINESTERASE AND ITS MOLECULAR FORMS IN PIG CEREBRAL CORTEXJournal of Neurochemistry, 1976
- Preparation of rat liver cellsExperimental Cell Research, 1973
- A new procedure for the extraction, purification and fractionation of brain gangliosidesBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1973
- Axonal Transport of Gangliosides in the Goldfish Optic NerveScience, 1972
- HIGH-YIELD PREPARATION OF ISOLATED RAT LIVER PARENCHYMAL CELLSThe Journal of cell biology, 1969
- Isolation of rat-liver lysosomes and their general propertiesBiochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects, 1964
- Quantitive estimation of sialic acidsBiochimica et Biophysica Acta, 1957