ANAEROBIC CONVERSION OFD-XYLOSE TO TRIOSE PHOSPHATE AND HEXOSE PHOSPHATE BY EXTRACTS OF PSEUDOMONAS HYDROPHILA

Abstract

By analysis of reaction mixtures containing D-xylose, adenosinetriphosphate, and cell-free sonic extracts of Pseudomonas hydrophila, evidence has been obtained for the formation of triose and hexose phosphate is under anaerobic conditions. The hexose phosphate fraction was shown to be a mixture of fructose-6-phosphate and glucose-6-phosphate by chromatographic and by enzymatic methods. The time course of the formation and disappearance of pentose, heptulose, triose, and hexose phosphate intermediates was also determined. These data, together with the results of previous work from this laboratory, have been combined to present an over-all scheme of enzymatic reactions for the anaerobic conversion of D-xylose to triose and hexose phosphates.