A simple method of optical resolution of racemic drugs was developed by a combination of cyclodextrin complexation and subsequent Sephadex gel chromatography. Chiral discrimination with cyclodextrins was assessed by circular dichroism measurement. The optical purities of enantiomers separated by this method were above 40% and 30% for warfarin and mandelic acid, respectively. Our results demonstrate the utility of this method for easy resolution of racemates.