Forskolin Stimulates Adenylate Cyclase Activity, Adenosine 3′,5′-Monophosphate Production and Peptide Release from the Intermediate Lobe of the Rat Pituitary Gland
- 1 March 1984
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 114 (3), 761-766
- https://doi.org/10.1210/endo-114-3-761
Abstract
Forskolin stimulates adenylate cyclase activity in a cell-free homogenate of the intermediate lobe (IL) of the rat pituitary gland. l-Isoproterenol, a β-adrenergic agonist, enhances and apomorphine, a D-2 agonist, inhibits the forskolinstimulated adenylate cyclase activity. Guanosine triphosphate is obligatory for the dopaminergic attenuation of the forskolinenhanced enzyme activity. When tested upon enzymatically dispersed IL cells, forskolin enhances the release of both cAMP and immunoreactive αMSH-like peptides. These effects of forskolin upon the IL are discussed within the context of a previously published working hypothesis that cAMP in some way triggers the calcium-dependent release of hormones from the IL. (Endocrinology114: 761, 1984)Keywords
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