Histochemical identification of human T lymphocytes from paraffin sections.
Open Access
- 1 July 1980
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 28 (7), 704-707
- https://doi.org/10.1177/28.7.6156204
Abstract
The alpha-naphthyl acetate esterase (ANEA) is a histochemical marker for human T lymphocytes in cell smears and frozen tissue sections. We have now applied the ANAE method to paraffin-embedded tissue sections. We first demonstrated with cytocentrifuged cell smears of blood leukocytes that the ANAE activity is preserved upon prolonged storage in formol calcium, Holt's buffer, acetone, xylene, and heat. When the tissue sections were similarly processed and embedded in paraffin, the ANAE positive (T) lymphocytes were identified by their distinct display of one or more reddish-brown reaction dots in the cell cytoplasm. ANAE positive mononuclear phagocytes were easioy distinguished from the T lymphocytes by their diffuse, sodium fluoride-sensitive pancytoplasmic reaction. The extension of the ANAE method to paraffin-embedded tissue sections with superior morphological integrity, makes it possible to apply it in practical biopsy pathology.This publication has 7 references indexed in Scilit:
- Nonspecific esterase activity in human lymphocytesClinical Immunology and Immunopathology, 1978
- CYTOCHEMICAL IDENTIFICATION OF HUMAN T-LYMPHOCYTES1978
- ACCUMULATION OF THYROID ANTIGEN-REACTIVE T-LYMPHOCYTES IN GLAND OF PATIENTS WITH SUB-ACUTE THYROIDITIS1978
- Acid Esterase in Human Lymphoid Cells and Leukaemic Blasts: a Marker for T LymphocytesBritish Journal of Haematology, 1977
- Expression of the Acid α‐Naphthyl Acetate Esterase Marker by Activated and Secondary T Lymphocytes in ManScandinavian Journal of Immunology, 1977
- Identification of Resting Human T and B Lymphocytes by Acid α‐Naphthyl Acetate Esterase Staining Combined with Rosette Formation with Staphylococcus aureus Strain Cowan 1Scandinavian Journal of Immunology, 1976
- Predominance of t cells in the lymphocytic infiltrates of synovial tissues in rheumatoid arthritisArthritis & Rheumatism, 1976