Immunological Relationships among Embryonic and Adult Chicken Pepsinogens: A Study with Monoclonal and Polyclonal Antibodies
Open Access
- 31 January 1987
- journal article
- research article
- Published by Wiley in Development, Growth & Differentiation
- Vol. 29 (1), 85-91
- https://doi.org/10.1111/j.1440-169x.1987.00085.x
Abstract
To investigate the immunological relationships of pepsinogen isozymes present in embryonic and adult chicken proventriculi, we obtained monoclonal and polyclonal antibodies to these pepsinogens. Zymograms and immunoblots demonstrated that monoclonal antibody Y37 reacted with both embryonic and slow-migrating adult pepsinogens, while polyclonal antibodies against embryonic pepsinogen and fast-migrating adult pepsinogen were specific for these respective antigens. Shift from embryonic to adult-type pepsinogen occurred at about the time of hatching and the localizations of embryonic and adult-type pepsinogens within proventricular gland cells were found to differ by the indirect immunofluorescence method. Results with these antibodies revealed the immunological relations of these pepsinogens and the unique properties of embryonic chicken pepsinogen.This publication has 21 references indexed in Scilit:
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