Relaxation of muscle fibres by photolysis of caged ATP
- 1 December 1982
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 300 (5894), 701-705
- https://doi.org/10.1038/300701a0
Abstract
A novel method was developed for studying the reaction kinetics of the force-generating mechanism in [frog] muscle. Inert photolabile precursors of ATP or ADP are incorporated into muscle fibers having their surface membrane barrier removed. The nucleotide is then rapidly liberated by laser pulse photolysis. This circumvents the limitation in time resolution set by diffusion of nucleotide from the medium bathing the fiber. This laser photolysis method may be applicable to studies of the dynamic properties of many biological systems.This publication has 30 references indexed in Scilit:
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