The sulphatase of ox liver. 3. Further observations on sulphatase B and an investigation of the origin of fractions A and B
- 1 July 1954
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 57 (3), 465-470
- https://doi.org/10.1042/bj0570465
Abstract
A method is described for the preparation of sulfatase B from acetone-dried liver powder by repeated fractional precipitation with acetone. The Km value for nitro-catechol sulfate is 0.06-0.08 M The optimum pH is 5.7. Citrate buffers give considerable inhibition. Na2SO4 and Na2SO3 are inhibitors but not NaCl, KCN and inhibitors of liver esterase. The sulfatase can be separated from phosphatase and esterase by paper electrophoresis. Both sulfatases were shown to be present in aqueous extracts from fresh ox and rat livers.Keywords
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