Identification of Glycoproteins from Mouse Skin Tumors and Plasma
Open Access
- 4 September 2008
- journal article
- research article
- Published by Springer Nature in Clinical Proteomics
- Vol. 4 (3-4), 117-136
- https://doi.org/10.1007/s12014-008-9014-z
Abstract
Introduction: Plasma has been the focus of testing different proteomic technologies for the identification of biomarkers due to its ready accessibility. However, it is not clear if direct proteomic analysis of plasma can be used to discover new marker proteins from tumors that are associated with tumor progression. In this paper, we reported that such proteins can be detected in plasma in a chemical-induced skin cancer model in mice. Materials and Methods: We analyzed glycoproteins from both benign papillomas and malignant carcinomas from mice using our recently developed platform, solid-phase extraction of glycopeptides and mass spectrometry, and identified 463 unique N-linked glycosites from 318 unique glycoproteins. These include most known extracellular proteins that have been reported to play roles in skin cancer development such as thrombospondin, cathepsins, epidermal growth factor receptor, cell adhesion molecules, cadherins, integrins, tuberin, fibulin, and TGFβ receptor. We further investigated whether these tumor proteins could be detected in plasma from tumor-bearing mice using isotope labeling and 2D liquid chromatography/matrix-assisted laser desorption/ionization tandem mass spectrometry. Results and Discussion: Two tumor glycoproteins, Tenascin-C and Arylsulfatase B, were identified and quantified successfully in plasma from tumor bearing mice. This result indicates that analysis of tumor-associated proteins in tumors and plasma by a method using glycopeptide capture, isotopic labeling, and mass spectrometry can be used as a discovery tool to identify candidate tumor proteins that may be detected in plasma.Keywords
This publication has 31 references indexed in Scilit:
- High Sensitivity Detection of Plasma Proteins by Multiple Reaction Monitoring of N-GlycositesMolecular & Cellular Proteomics, 2007
- Human sulfatases: A structural perspective to catalysisCellular and Molecular Life Sciences, 2007
- High Throughput Proteome Screening for Biomarker DetectionMolecular & Cellular Proteomics, 2005
- Potential oncogenic action of tenascin-C in tumorigenesisThe International Journal of Biochemistry & Cell Biology, 2005
- Chemical probes and tandem mass spectrometry: a strategy for the quantitative analysis of proteomes and subproteomesCurrent Opinion in Chemical Biology, 2003
- Characterization of the Low Molecular Weight Human Serum ProteomeMolecular & Cellular Proteomics, 2003
- The case for early detectionNature Reviews Cancer, 2003
- Empirical Statistical Model To Estimate the Accuracy of Peptide Identifications Made by MS/MS and Database SearchAnalytical Chemistry, 2002
- ProteinN-Glycosylation along the Secretory Pathway: Relationship to Organelle Topography and Function, Protein Quality Control, and Cell InteractionsChemical Reviews, 2002
- The tenascin family of ECM glycoproteins: Structure, function, and regulation during embryonic development and tissue remodelingDevelopmental Dynamics, 2000