Syntabulin, a motor protein linker, controls dorsal determination
Open Access
- 15 March 2010
- journal article
- Published by The Company of Biologists in Development
- Vol. 137 (6), 923-933
- https://doi.org/10.1242/dev.046425
Abstract
In amphibian and teleost embryos, the dorsal determinants (DDs) are believed to be initially localized to the vegetal pole and then transported to the prospective dorsal side of the embryo along a microtubule array. The DDs are known to activate the canonical Wnt pathway and thereby promote the expression of genes that induce the dorsal organizer. Here, by identifying the locus of the maternal-effect ventralized mutant tokkaebi, we show that Syntabulin, a linker of the kinesin I motor protein, is essential for dorsal determination in zebrafish. We found that syntabulin mRNA is transported to the vegetal pole during oogenesis through the Bucky ball (Buc)-mediated Balbiani body-dependent pathway, which is necessary for establishment of animal-vegetal (AV) oocyte polarity. We demonstrate that Syntabulin is translocated from the vegetal pole in a microtubule-dependent manner. Our findings suggest that Syntabulin regulates the microtubule-dependent transport of the DDs, and provide evidence for the link between AV and dorsoventral axis formation.Keywords
This publication has 71 references indexed in Scilit:
- Vegetally localized Xenopus trim36 regulates cortical rotation and dorsal axis formationDevelopment, 2009
- Bucky ball functions in Balbiani body assembly and animal–vegetal polarity in the oocyte and follicle cell layer in zebrafishDevelopmental Biology, 2008
- Maternal nodal and zebrafish embryogenesisNature, 2007
- Viral 2A peptides allow expression of multiple proteins from a single ORF in transgenic zebrafish embryosGenesis, 2007
- Time-dependent patterning of the mesoderm and endoderm by Nodal signals in zebrafishBMC Developmental Biology, 2007
- nanos1 is required to maintain oocyte production in adult zebrafishDevelopmental Biology, 2007
- The zebrafish dorsal axis is apparent at the four-cell stageNature, 2005
- Splicing of oskar RNA in the nucleus is coupled to its cytoplasmic localizationNature, 2004
- A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applicationsNature Biotechnology, 2002
- Silberblick/Wnt11 mediates convergent extension movements during zebrafish gastrulationNature, 2000