Section Thickness and Grain Count Variation in Tritium Autoradiography

Abstract

H2 styrene-glycolmethacrylate is a suitable standard source for autoradiographic model studies. When it is used for quantitative autoradiography, the coefficient of variation for grain counts is minimal at a section thickness of 2.5 μ and greater. Variation increases with progressively thinner sections. When sections of the same thickness are mounted on separate slides, there is no significant variation in grain counts at the 95% confidence level when the slides are processed simultaneously.