Effects of Glucose Dehydrogenase in Converting Urea and Ammonia into Amino Acid Using Artificial Cells

Abstract
A microencapsulated multienzyme system containing urease, glutamate dehydrogenase and glucose dehydrogenase was used to convert urea and ammonia into an amino acid. The effect of 2 different glucose dehydrogenases was studied in detail. High-specific-activity glucose dehydrogenase requires minimal cofactor and glucose and can greatly facilitate the further development of this approach for possible clinical applications.