The Effect of Acceptor Oligoribonucleotide Sequence on the T4 RNA Ligase Reaction
Open Access
- 1 July 1982
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 125 (3), 639-643
- https://doi.org/10.1111/j.1432-1033.1982.tb06730.x
Abstract
In order to make efficient use of T4 RNA ligase in a program involving chemical-enzymatic oligoribonucleotide synthesis, the sequence effects of the acceptor oligomer have been refined. The reaction of the donor molecules pCp and pUpUpUpCp with a series of trinucleoside diphosphates ApApN, CpNpA and NpCpA (where N = U, C, A or G) was examined. High-performance liquid chromatography was used for simultaneous analysis of all substrates, intermediates and products. For all trinucleoside bisphosphates tested a substantial amount of the intermediate A(5′)pp(5′)Cp or A(5′)pp(5′)UpUpUpCp was observed. This indicated that the extent of ligation was dependent upon sequence of the acceptor molecule and not upon adenylation of the donor. Con- versely, examination of the four nucleoside 3′,5′-bisphosphate donors p u p, pCp, pAp and pGp with a common acceptor ApApU indicated that in the case of poor ligation little of the intermediate adenylated donor was formed.This publication has 12 references indexed in Scilit:
- RNA ligase reaction products in plasmolyzed Escherichia coli cells infected by T4 bacteriophage.Proceedings of the National Academy of Sciences, 1979
- T4 RNA ligase joins 2'-deoxyribonucleoside 3',5'-bisphosphates to oligodeoxyribonucleotidesBiochemistry, 1978
- Enzymic oligoribonucleotide synthesis with T4 RNA ligaseBiochemistry, 1978
- Addition of mononucleotides to oligoribonucleotide acceptors with T4 RNA ligase.Proceedings of the National Academy of Sciences, 1978
- Dinucleoside pyrophosphate are substrates for T4-induced RNA ligase.Proceedings of the National Academy of Sciences, 1977
- Oligoribonucleotide synthesis. X. An improved synthesis of the anticodon loop region of methionine transfer ribonucleic acid from E. coliCanadian Journal of Chemistry, 1976
- Determination of recognition sites of T4 RNA ligase on the 3′-OH and 5′-P termini of polyribonucleotide chainsNature, 1975
- T4-induced RNA ligase joins single-stranded oligoribonucleotides.Proceedings of the National Academy of Sciences, 1975
- Oligoribonucleotide synthesis. VII. Synthesis of the anticodon loop of Escherichia coli methionine transfer ribonucleic acidJournal of the American Chemical Society, 1974
- Oligoribonucleotide Synthesis. IV. Approach to Block SynthesisCanadian Journal of Chemistry, 1972