Ultrastructural localization of carbonic anhydrase in the chorioallantoic membrane by immunocytochemistry.
- 1 October 1981
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 29 (10), 1121-1127
- https://doi.org/10.1177/29.10.6170666
Abstract
Carbonic anhydrase was localized in the chick embryo chorionic ectoderm at the ultrastructural level by immuno-cytochemistry. Preembedding staining of whole tissue was performed. The enzyme was present in the cytoplasm, on the membranes of apical vesicles, and on the membranes of microvilli in villus cavity cells, cells that may be involved in acid secretion and subsequent dissolution of the egg shell. In sinus covering cells, the enzyme is solely in the cytoplasm. The location of the enzyme in the thin cytoplasmic arms of the sinus covering cells is consistent with the role in nonrespiratory CO2 release.This publication has 15 references indexed in Scilit:
- Functional involvement of carbonic anhydrase in calcium transport of the chick chorioallantoic membraneBiochemical Journal, 1978
- Ultrastructural observations on the chorionic epithelium, parathyroid glands and bones from chick embryos developed in shell-less cultureDevelopment, 1978
- A Comparison of Methods Using Diaminobenzidine (Dab) to Localize Peroxidases in Erythrocytes, Neutrophils, and Peroxidase-Antiperoxidase ComplexStain Technology, 1978
- STRUCTURE OF INTRA-CHORIONIC BLOOD SINUS IN CHICK-EMBRYO1977
- Carbonic anhydrase activity of isolated brush border and basal-lateral membranes of renal tubular cellsPflügers Archiv - European Journal of Physiology, 1977
- ENZYME-LABELED ANTIBODIES FOR THE LIGHT AND ELECTRON MICROSCOPIC LOCALIZATION OF TISSUE ANTIGENSThe Journal of cell biology, 1967
- Histochemical demonstration of carbonic anhydrase activityHistochemistry and Cell Biology, 1967
- Organic-inorganic interrelationships in avian egg shellExperimental Cell Research, 1963
- CHORIO-ALLANTOIS OF CHICK - LIGHT AND ELECTRON MICROSCOPIC OBSERVATION AT VARIOUS TIME OF INCUBATION1963
- Inhibition of Gastric Secretion of Acid in Dogs by Carbonic Anhydrase Inhibitor, 2-Acetylamino-1,3,4-Thiadiazole-5-SulfonamideAmerican Journal of Physiology-Legacy Content, 1952