MODULATION AND DYNAMICS OF PHASE PROPERTIES IN PHOSPHOLIPID MIXTURES DETECTED BY LAURDAN FLUORESCENCE*
Open Access
- 1 March 1993
- journal article
- Published by Wiley in Photochemistry and Photobiology
- Vol. 57 (3), 403-410
- https://doi.org/10.1111/j.1751-1097.1993.tb02309.x
Abstract
Steady-state and dynamic fluorescence properties of 6-lauroyl-2-dimethylaminonaphthalene (Laurdan) have been used to ascertain the coexistence of separate phase domains and their dynamic properties in phospholipid vesicles composed of different mole ratios of dilauroyl- and dipalmitoyl-phosphatidylcholine (DLPC and DPPC, respectively). The recently introduced generalized polarization together with time-resolved emission spectra have been utilized for detecting changes. The results indicate the coexistence of phospholipid phase domains in vesicle compositions in the range between 30 mol% and 70 mol% DPPC in DLPC. Below and above these concentrations a homogeneous phase is observed, with averaged properties. In the case of coexisting phase domains, the properties of each individual phase are largely influenced by the presence of the other phase. Implications on fluctuations between the coexisting phases and on the size and shape of domains are discussed.Keywords
This publication has 22 references indexed in Scilit:
- Density fluctuations in saturated phospholipid bilayers increase as the acyl-chain length decreasesBiophysical Journal, 1990
- The effect of HIV-1 infection on the lipid fatty acid content in the membrane of cultured lymphocytesAIDS, 1990
- Regulation of chloride transport in parotid secretory granules by membrane fluidityBiochemistry, 1990
- Effect of membrane fatty acyl composition on LDL metabolism in Hep G2 hepatocytesBiochemistry, 1990
- Lateral diffusivity of lipid analogue excimeric probes in dimyristoylphosphatidylcholine bilayersBiophysical Journal, 1990
- Localization of the Pancreatic Beta Cell Glucose Transporter to Specific Plasma Membrane DomainsScience, 1989
- Lipid sorting in epithelial cellsBiochemistry, 1988
- Thermodynamic properties of the lipid bilayer transition. Pseudocritical phenomenaBiophysical Journal, 1983
- FLUOROPHORES IN POLAR MEDIA: SPECTRAL EFFECTS OF THE LANGEVIN DISTRIBUTION OF ELECTROSTATIC INTERACTIONSAnnals of the New York Academy of Sciences, 1981
- Lipid phase transitions and phase diagrams I. Lipid phase transitionsBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1977