A Thin Gel Quantitative Two-Dimensional Immunoelectrophoretic Method

Abstract

Firestone, Harvey J., and Aronson, Samuel B.: A thin gel quantitative twodimensional immunoelectrophoretic method. Techn. Bull. Regist. Med. Techn., 39: 217–224, 1969. A quantitative, two-dimensional, thin agarose gel immunoelectrophoretic method capable of separating 30 proteins is described. The method has improved rapidity (3 to 4 hr.), sensitivity (to 30 ng.), and economy of antiserum and increased capacity to eight analytic patterns per plate. In an 8-hr. period, 64 patterns have been produced. Protein peak identification and quantitation are simplified with the use of a standardized human serum containing known concentrations of 17 proteins. Linear measurements of each symmetrical peak are converted to peak areas, which are proportional to the corresponding protein concentration. However, the method is not suited to evaluation of the immunoglobulins.