The activity of phosphoenolpyruvate carboxykinase in rat tissues. Assay techniques and effects of dietary and hormonal changes
- 15 November 1975
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 152 (2), 401-408
- https://doi.org/10.1042/bj1520401
Abstract
1. Phosphoenolpyruvate carboxykinase was assayed by three methods: (i) incorporation of H14CO3- into oxaloacetate: (ii) conversion of oxaloacetate into phosphoenolpyruvate, subsequently assayed enzymically; and (iii) transfer of 32P from [γ-32P]GTP to oxaloacetate. 2. Enzyme activity is increased in liver and epididymal adipose tissue in alloxan-diabetes and starvation, and in kidney in starved, acidotic and steroid-treated animals. 3. The ratios of the ‘back’ to the ‘forward’ reactions in liver, kidney and epididymal adipose tissue are different and characteristic of each tissue; they differ markedly from values reported for the purified mitochondrial enzyme. 4. The ratio of the ‘back’ to ‘forward’ reaction in any one tissue is constant in adrenalectomized, diabetic, acidotic and steroid-treated animals. 5. In starved animals, the ratio is increased in liver and kidney, but decreased in epididymal adipose tissue. 6. Administration of l-tryptophan results in an acute (1h) increase in activity measured in the ‘forward’ direction alone in liver and epididymal adipose tissue, but not in kidney.Keywords
This publication has 40 references indexed in Scilit:
- The Localization of Gluconeogenesis in Rat Nephron. Determination of Phosphoenolpyruvate Carboxykinase in Microdissected TubulesBiological Chemistry, 1974
- In vitro stimulation of gluconeogenesis from pyruvate and of 14CO2-fixation by dexamethasone phosphate and vasopressin in the kidney cortex of adrenalectomized ratsBiochemical and Biophysical Research Communications, 1972
- The effect of steroids and ammonium chloride acidosis on phosphoenolpyruvate carboxykinase in rat kidney cortexJCI Insight, 1972
- The effect of steroids and ammonium chloride acidosis on phosphoenolpyruvate carboxykinase in rat kidney cortexJCI Insight, 1972
- Induction of phosphoenolpyruvate carboxykinase by N6,O2′-dibutyryl cyclic amp in rat liverBiochimica et Biophysica Acta (BBA) - General Subjects, 1972
- Aspects of tryptophan metabolism in health and disease: a review.Journal of Clinical Pathology, 1972
- Response of Phosphopyruvate Carboxylase to Tryptophan Metabolites and Metal IonsEuropean Journal of Biochemistry, 1971
- A reinvestigation of the kinetic parameters of phosphoenolpyruvate carboxykinaseBiochemical and Biophysical Research Communications, 1971
- Chicken Liver Mitochondrial Phosphoenolpyruvate CarboxykinaseEuropean Journal of Biochemistry, 1970
- Renal metabolic response to acid base changesJCI Insight, 1969