An Improved Chromatographic Procedure for Quantitation of Human Fetal Hemoglobin

Abstract
The DEAE-cellulose chromatographic procedure which uses glycine-KCN-NaCl solutions as developers and a NaCl gradient to elute the Hb zones was useful for the quantitation of Hb F in samples containing Hb A, provided the level of Hb F exceeds 2%. The method was evaluated through a study of artificial mixtures and of blood samples from patients with various disorders. The data were compared with results obtained by 4 other procedures that are used for the quantitation of Hb F.