Nucleotide binding of the C‐terminal domains of the major histocompatibility complex‐encoded transporter expressed in Drosophila melanogaster cells
- 22 August 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 350 (2-3), 337-341
- https://doi.org/10.1016/0014-5793(94)00806-x
Abstract
The C-terminal domains of the mouse transporter associated with antigen processing (TAP) were expressed as soluble proteins in Drosophila melanogaster cells and labeled by [α-32P]8-azido-ATP after UV-irradiation. The relative potencies of the nucleotides in preventing azido-ATP labeling were in the order of ATP > GTP > CTP > ITP > UTP for both the TAP1 and TAP2 C-terminal domains, suggesting ATP to be the natural substrate of the transporter. Our data provide the first evidence that the individual C-terminal domain of either TAP1 or TAP2 can be expressed as a functional ATP-binding proteinKeywords
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