The fate of acetyl groups derived from glucose in the isolated perfused goat udder
- 1 April 1966
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 99 (1), 228-231
- https://doi.org/10.1042/bj0990228
Abstract
In the isolated perfused goat mammary gland, both glucose and acetate contribute 20-30% of carbon to citrate via acetyl-CoA. Carbon dioxide does not contribute significantly to the formation of acetyl-CoA. Adenosine triphosphate citrate lyase, which, in rats, yields acetyl-CoA for fatty acid synthesis, is absent from or very low in high-speed supernatant fraction of ruminant tissue. It is reasonable to infer that intramitochondrial acetyl-CoA, to which both glucose and acetate contribute, is separated from extramitochondrial acetyl-CoA to which glucose does not contribute and which is used for fatty acid synthesis.This publication has 16 references indexed in Scilit:
- CITRATE AND THE CONVERSION OF CARBOHYDRATE INTO FAT. THE ACTIVITIES OF CITRATE-CLEAVAGE ENZYME AND ACETATE THIOKINASE IN LIVERS OF STARVED AND RE-FED RATSBiochemical Journal, 1965
- CO2 formed from endogenous and exogenous substrates in perfused rat heartAmerican Journal of Physiology-Legacy Content, 1964
- Citrate and the conversion of carbohydrate into fat. A comparison of citrate and acetate incorporation into fatty acidsBiochemical Journal, 1964
- The metabolism of acetate and glucose by the isolated perfused udder. 2. The contribution of acetate and glucose to carbon dioxide and milk constituentsBiochemical Journal, 1963
- The Supply of Precursors for the Synthesis of Fatty AcidsJournal of Biological Chemistry, 1962
- Incorporation of radioactive citrate into fatty acidsBiochimica et Biophysica Acta, 1962
- Chemical degradation of 14C-labeled glutamic acidBiochimica et Biophysica Acta, 1961
- Biochemical aspects of bovine ketosisBiochemical Journal, 1959
- CITRATE CLEAVAGE ENZYME .1. DISTRIBUTION AND PURIFICATION1959
- Methods for Determination of Coenzyme APublished by Wiley ,1955