A putative HCO−3 transporter in the cyanobacterium Synechococcus sp. strain PCC 79421
- 3 July 1998
- journal article
- Published by Wiley in FEBS Letters
- Vol. 430 (3), 236-240
- https://doi.org/10.1016/s0014-5793(98)00662-0
Abstract
Cyanobacteria possess an inducible mechanism which enables them to concentrate inorganic carbon (Ci) within the cells. An inactivation library was used to raise the high-CO2-requiring mutant of Synechococcus PCC 7942, IL-2, impaired in HCO− 3 transport. Analysis of the relevant genomic DNA detected several modifications, probably due to the single crossover recombination, leading to inactivation of ORF467 (designated ictB) in IL-2. IctB contains 10 trans-membrane regions and is homologous to several transport-related proteins from various organisms. Kinetic analyses of HCO− 3 uptake in the wild type and IL-2 suggested the presence of two or three HCO− 3 carriers exhibiting different affinities to HCO− 3.Keywords
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