Histone protein and DNA synthesis in HeLa cells after thermal shock
- 1 February 1984
- journal article
- research article
- Published by Wiley in Journal of Cellular Physiology
- Vol. 118 (2), 153-160
- https://doi.org/10.1002/jcp.1041180207
Abstract
Exposure of suspension‐cultured HeLa cells to a 45° thermal shock resulted in cell inactivation and inhibition of both protein and DNA synthesis. DNA synthesis was inhibited in a biphasic manner with a more sensitive (D0 = 7 min) and a less sensitive (D0 = 20 min) phase. The less sensitive process was demonstrated to be DNA chain elongation. Transport of thymidine into intracellular pools was significantly less sensitive to thermal shock (D0 in excess of 200 min). When HeLa cells were heated at 45° for 15 min there was an 80% inhibition of incorporation of precursors into both DNA and protein with little effect on precursor transport into cellular pools. While the rate of synthesis of whole cell and histone protein (H2a, H2b, H3, and H4) and DNA chain elongation recovered by 6 h after cell heating, total precursor incorporation into DNA was only 0.4 of control levels. The long‐term depression of the DNA synthetic rate could not be explained by a cell cycle redistribution, a depression in the total fraction of S phase cells synthesizing DNA, or by a depression in the rate of DNA chain elongation. We conclude that thermal shock results in a long‐term depression in the fraction of cell replicons involved in DNA replication.This publication has 25 references indexed in Scilit:
- FACTORS REGULATING MEMBRANE PERMEABILITY ALTER THERMAL RESISTANCE*Annals of the New York Academy of Sciences, 1980
- The initiation and elongation steps in protein synthesis: Relative rates in chinese hamster ovary cells during and after hyperthermic and hypothermic shocksJournal of Cellular Physiology, 1979
- Thermal denaturation of nucleosomal core particlesNucleic Acids Research, 1978
- Cooperative alignment of nu bodies during chromosome replication in the presence of cycloheximideCell, 1976
- Formation of nascent DNA molecules during inhibition of replicon initiation in mammalian cellsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1976
- The biochemical mechanism of selective heat sensitivity of cancer cells: II. Studies on nucleic acids and protein synthesisEuropean Journal of Cancer (1965), 1969
- Regulation of protein synthesis in HeLa cells: Translation at elevated temperaturesJournal of Molecular Biology, 1969