Calcium mobilisation controls tyrosine protein phosphorylation independently of the activation of protein kinase C in human platelets

Abstract

We have investigated the regulation of tyrosine proteins phosphorylation by intracellular Ca²⁺ level ([Ca²⁺]_i) and protein kinase C (PKC) during platelet stimulation. We found that chelation of extracellular calcium completely prevented phosphorylation of tyrosine proteins induced by thapsigargin and phorbol 12-myristate 13-acetate (PMA), whereas, when induced by thrombin, it prevented a subset of tyrosine proteins. The selective inhibition of PKC by OF 109203X did not abolish tyrosine protein phosphorylation when induced by thrombin and thapsigargin. The results suggest that in human platelets tyrosine protein phosphorylation is dependent on [Ca²⁺]_i, although direct PKC activation can also induce phosphorylation of tyrosine proteins.