Modified photometric method for the determination of phospholipase A activities
- 1 February 1989
- journal article
- research article
- Published by Springer Nature in Klinische Wochenschrift
- Vol. 67 (3), 106-109
- https://doi.org/10.1007/bf01711332
Abstract
A modified photometric method for the determination of phospholipase A activities which is based on a previously published reaction principle is described. The modified assay uses a lyophilized substrate emulsion rather than a freshly prepared phospholipid emulsion and a new chromogen (tribromohydroxybenzoic acid and 4-amino-antipyrine) with a high molar absorption coefficient (1,930 m2/mol at 546 nm, 2,900 m2/mol at 512 nm wavelength). The new test is more practicable with respect to pipetting volumes and incubation times. Preliminary results of a method evaluation indicate that the modified assay fulfills the usual criteria for clinical chemical enzyme measurements.Keywords
This publication has 16 references indexed in Scilit:
- Rapid determination of phospholipase A in human serum and plasma.Clinical Chemistry, 1987
- An enzymatic colorimetric assay of calcium-dependent phospholipases AAnalytical Biochemistry, 1987
- Activity of phospholipase A compared in serum of patients with pancreatic and nonpancreatic diseases.Clinical Chemistry, 1987
- The PhospholipasesPublished by Springer Nature ,1987
- An enzymatic method for calibration of serum lipase assays.Clinical Chemistry, 1986
- Photometric Determination of Phospholipase Acclm, 1986
- Fluorometric assay for phospholipase A2 in serum.Clinical Chemistry, 1985
- Phospholipase A2 activity of post-heparin plasma: A rapid and sensitive assay and partial characterizationAnalytical Biochemistry, 1981
- Evidence for active phospholipase a in mouse plasmaBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1980
- MEASUREMENT OF LECITHINASE A IN SERUM AND OTHER BODY FLUIDS1961