Purification of the Ca2+-and Mg2+-requiring ATPase from rat brain synaptic plasma membrane
- 1 November 1982
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 207 (2), 225-231
- https://doi.org/10.1042/bj2070225
Abstract
A Ca2+-ATPase (Ca2+- and Mg2+-requiring ATPase) was purified from a synaptic plasma-membrane fraction of rat brain. This enzyme had properties similar to those of plasma-membrane Ca2+-ATPases from other organs: its splitting of ATP was dependent on both Ca2+ and Mg2+, it bound in a Ca2+-dependent fashion to calmodulin-Sepharose and it cross-reacted with specific antibodies raised against human erythrocyte-membrane Ca2+-ATPase. It had an apparent Mr of 138 000, similar to those of plasma-membrane ATPases from human erythrocyte and from dog heart sarcolemma. Previous high-Ca2+-affinity ATPases observed in brain had Mr 100 000; in at least one case, such an ATPase probably represented a different type of enzyme, derived from coated vesicles.This publication has 22 references indexed in Scilit:
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