Cell-Free Nitrogenase and Hydrogenase from Actinorhizal Root Nodules

Abstract
Field-grown alder (Alnus glutinosa) root nodules were disrupted in liquid N2 to release the actinomycete endophytes [Agotobacter vinelandii]. The endophytes were broken by mild sonic oscillation and yielded a cell-free nitrogenase preparation capable of reducing acetylene and protons. In addition, the preparation carried a cell-free uptake hydrogenase.