Kinase-Catalyzed Modification of Gold Nanoparticles: A New Approach to Colorimetric Kinase Activity Screening
- 31 January 2006
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 128 (7), 2214-2215
- https://doi.org/10.1021/ja058135y
Abstract
Peptide-stabilized gold nanoparticles have been enzymatically biotinylated by a kinase-catalyzed reaction using biotin-ATP as a cosubstrate. Upon mixing with avidin-modified particles, solutions of biotinylated particles change color from red to blue, indicating aggregation of particles. On the basis of this reaction, we have developed a simple colorimetric test to monitor kinase inhibitor activity.Keywords
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