Purification of Naked Intranuclear Particles from Human Liver Infected by Hepatitis B Virus
Open Access
- 1 September 1974
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 71 (9), 3345-3349
- https://doi.org/10.1073/pnas.71.9.3345
Abstract
The isolation and purification of the naked intranuclear particles of a human liver infected with hepatitis B virus is described. The particles had a diameter of 27-28 nm, were hexagonal, and showed the presence of capsomeres and spikes with lengths of 7-10 nm projecting from the surfaces. The particles formed cubic arrays with center-to-center distances of 40-42 nm. The overall particle symmetry was icosahedral. The ultraviolet absorption spectrum of the particles showed a peak at 264 nm with a shoulder at 280 nm. Endogenous DNA polymerase activity was not detected in fractions containing particles. DNA polymerase activity was present when activated DNA or d(A-T)(n) was used as exogenous template.Keywords
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